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Image Search Results
Journal: ACS infectious diseases
Article Title: Direct-qPCR Assay for Coupled Identification and Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae
doi: 10.1021/acsinfecdis.8b00104
Figure Lengend Snippet: Use of direct-qPCR assay for quantitation of N. gonorrhoeae in GW medium. (A) Direct-qPCR quantifies cells ranging from 107 CFU/mL to 102 CFU/mL. Cq values were obtained using regression fit determined by the BioRad CFX Manager v3.1. Amplification curves are highly consistent across technical replicates, although some stochastic effects were observed when only a few target molecules (102 CFU/mL) are present in the sample. (B) Linear fit of the quantitative Cq values using Origin Pro 2017 with instrumental weighting. PCR efficiency of approximately 100% was obtained from the slope value of the linear fit.
Article Snippet: Cq values were obtained using regression fit determined by the
Techniques: Quantitation Assay, Amplification